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Importance of pulsing illumination parameters in low-level-light therapy

Source: J. Biomed. Opt. 15, 048005 (2010); doi:10.1117/1.3477186

Published 10 August 2010

KEYWORDS and PACS
Keywords
PACS
PUBLICATION DATA
ISSN:
1553-9628 (online)
Publisher:
AIP is a member of CrossRef SPIE
Daniel Barolet
RoseLab Skin Optics Laboratory, 3333 Graham Boulevard, Suite 206, Montreal, Quebec, Canada, H3R 3L5 and McGill University, Department of Medicine, Dermatology Division, Montreal, Quebec, Canada, H3A 1A1

Pascale Duplay and Hélène Jacomy
INRS-Institut Armand-Frappier, 531 boul. des Prairies, Laval, Québec, Canada, H7V 1B7

Mathieu Auclair
RoseLab Skin Optics Laboratory, 3333 Graham Boulevard, Suite 206, Montreal, Quebec, Canada, H3R 3L5
The influence of emission parameters in low-level-light therapy on cellular responses is not yet fully understood. This study assessed the impact of various light delivery modes on collagen production in human primary fibroblast cultured in monolayers after three treatments with red light-emitting diode illumination (630  nm, 8  J/cm2). Human type I collagen was measured in cell culture supernatants with procollagen type I C-peptide enzyme immunoassay. Results demonstrated that, 72  h post-baseline, specific microsecond pulsing patterns had a more favorable impact on the ability of fibroblasts to produce collagen de novo than comparative conditions of continuous wave, pulsed 50% duty cycle, and millisecond pulsing domains. The cascade of events leading to collagen production by red illumination may be explained by the photodissociation of nitric oxide from cytochrome c oxidase. Short and intermittent light delivery might enhance this cellular event. ©2010 Society of Photo-Optical Instrumentation Engineers
History: Received 30 March 2010; revised 22 June 2010; accepted 24 June 2010; published 10 August 2010
Permalink: http://dx.doi.org/10.1117/1.3477186

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