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Bubblegrams Reveal the Inner Body of Bacteriophage KZ

Source: Science 335, 182 (2012); http://dx.doi.org/10.1126/science.1214120

Issue Date: 15 January 2012

PUBLICATION DATA
ISSN:
1553-9628 (online)
Publisher:
AIP is a member of CrossRef AAAS
Weimin Wu,ff1 Julie A. Thomas,ff2 Naiqian Cheng,ff1 Lindsay W. Black,ff2 and Alasdair C. Stevenff1
ff1Laboratory of Structural Biology, National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS), National Institutes of Health, Bethesda, MD 20892, USA.
ff2Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, 108 North Greene Street, Baltimore, MD 21201, USA.
Dense packing of macromolecules in cellular compartments and higher-order assemblies makes it difficult to pick out even quite large components in electron micrographs, despite nominally high resolution. Immunogold labeling and histochemical procedures offer ways to map certain components but are limited in their applicability. Here, we present a differential mapping procedure, based on the physical principle of protein's greater sensitivity to radiation damage compared with that of nucleic acid. ©2012 American Association for the Advancement of Science

(As supplied by publisher.)

History: Received September 19, 2011; accepted December 02, 2011
Digital Object Identifier: http://dx.doi.org/10.1126/science.1214120
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