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Electrotaxis of lung cancer cells in ordered three-dimensional scaffolds

Source: Biomicrofluidics 6, 014102 (2012); http://dx.doi.org/10.1063/1.3671399

Published 4 January 2012

KEYWORDS and PACS
Keywords
PACS
  • 87.17.Jj
    Cell locomotion; chemotaxis
  • 87.50.cf
    Biophysical mechanisms of interaction (electric/magnetic fields)
  • 47.61.-k
    Micro- and nano-scale flow phenomena
  • 85.85.+j
    Micro- and nano-electromechanical systems (MEMS/NEMS) and devices
  • 87.80.Ek
    Mechanical and micromechanical techniques (biophysical research methods)
  • YEAR: 2011
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PUBLICATION DATA
ISSN:
1553-9628 (online)
Publisher:
AIP is a member of CrossRef AIP
Yung-Shin Sun,1 Shih-Wei Peng,1,1 Keng-Hui Lin,1,3 and Ji-Yen Cheng1,1,2
1Research Center for Applied Sciences, Academia Sinica, Taipei City 11529, Taiwan
2Institute of Biophotonics, National Yang-Ming University, Taipei City 11221, Taiwan
3Institute of Physics, Academia Sinica, Taipei City 11529, Taiwan
4Department of Mechanical and Mechantronic Engineering, National Taiwan Ocean University, Keelung 20224, Taiwan
In this paper, we report a new method to incorporate 3D scaffold with electrotaxis measurement in the microfluidic device. The electrotactic response of lung cancer cells in the 3D foam scaffolds which resemble the in vivo pulmonary alveoli may give more insight on cellular behaviors in vivo. The 3D scaffold consists of ordered arrays of uniform spherical pores in gelatin. We found that cell morphology in the 3D scaffold was different from that in 2D substrate. Next, we applied a direct current electric field (EF) of 338 mV/mm through the scaffold for the study of cells' migration within. We measured the migration directedness and speed of different lung cancer cell lines, CL1-0, CL1-5, and A549, and compared with those examined in 2D gelatin-coated and bare substrates. The migration direction is the same for all conditions but there are clear differences in cell morphology, directedness, and migration speed under EF. Our results demonstrate cell migration under EF is different in 2D and 3D environments and possibly due to different cell morphology and/or substrate stiffness. ©2012 American Institute of Physics
History: Received 23 September 2011; accepted 30 November 2011; published 4 January 2012
Digital Object Identifier: http://dx.doi.org/10.1063/1.3671399

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