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Cryptotomography: Reconstructing 3D Fourier Intensities from Randomly Oriented Single-Shot Diffraction Patterns

Source: Phys. Rev. Lett. 104, 225501 (2010); doi:10.1103/PhysRevLett.104.225501

Published 2 June 2010

PACS
  • 61.05.cf
    X-ray scattering (condensed matter structure determination)
  • 41.60.Cr
    Free-electron lasers
  • 42.30.Rx
    Phase retrieval
  • 42.55.Vc
    X- and γ-ray lasers
  • YEAR: 2010
PUBLICATION DATA
ISSN:
1553-9601 (online)
Publisher:
AIP is a member of CrossRef APS
N. D. Loh,1 M. J. Bogan,2 V. Elser,1 A. Barty,3 S. Boutet,2 S. Bajt,4 J. Hajdu,5 T. Ekeberg,5 F. R. N. C. Maia,5 J. Schulz,3 M. M. Seibert,5 B. Iwan,5 N. Timneanu,5 S. Marchesini,6 I. Schlichting,7,8 R. L. Shoeman,7,8 L. Lomb,7,8 M. Frank,9 M. Liang,3 and H. N. Chapman3,10
1Laboratory of Atomic and Solid State Physics Cornell University, Ithaca, New York 14853-2501, USA
2SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California, 94025, USA
3Center for Free-Electron Laser Science, DESY, Notkestrasse 85, Hamburg 22607, Germany
4Photon Science, DESY, Notkestrasse 85, Hamburg 22607, Germany
5Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3, Box 596, SE-75124 Uppsala, Sweden
6Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley California 94720, USA
7Max Planck Institute for Medical Research, Jahnstraße 29, 69120 Heidelberg, Germany
8Max Planck Advanced Study Group, Center for Free-Electron Laser Science, DESY, Notkestrasse 85, Hamburg 22607, Germany
9Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, California 94550, USA
10University of Hamburg, Luruper Chaussee 149, Hamburg 22761, Germany
We reconstructed the 3D Fourier intensity distribution of monodisperse prolate nanoparticles using single-shot 2D coherent diffraction patterns collected at DESY's FLASH facility when a bright, coherent, ultrafast x-ray pulse intercepted individual particles of random, unmeasured orientations. This first experimental demonstration of cryptotomography extended the expansion-maximization-compression framework to accommodate unmeasured fluctuations in photon fluence and loss of data due to saturation or background scatter. This work is an important step towards realizing single-shot diffraction imaging of single biomolecules. ©2010 The American Physical Society
History: Received 3 March 2010; published 2 June 2010
Permalink: http://link.aps.org/abstract/PRL/v104/e225501
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