Home | About Journal | Web Links | E-mail Alerts | RSS RSS Icon | Browse
Previous Article Next Article

Time-resolved imaging refractometry of microbicidal films using quantitative phase microscopy

Source: J. Biomed. Opt. 16, 120510 (2012); http://dx.doi.org/10.1117/1.3665439

Published 19 December 2011

KEYWORDS and PACS
Keywords
PACS
  • 87.64.M-
    Optical microscopy in biophysics and medical physics
  • 64.75.Bc
    Solubility
  • 87.19.xd
    Viral diseases
  • 07.60.Hv
    Optical refractometers and reflectometers
  • 78.47.jg
    Time resolved reflection spectroscopy
  • YEAR: 2011
PUBLICATION DATA
ISSN:
1553-9601 (online)
Publisher:
AIP is a member of CrossRef SPIE
Matthew T. Rinehart,1 Tyler K. Drake,1 Francisco E. Robles,1 Lisa C. Rohan,2 David Katz,3 and Adam Wax1
1Duke University, Department of Biomedical Engineering, Fitzpatrick Institute for Photonics, 3000 Science Drive, 136 Hudson Hall, Box 90281, Durham, North Carolina 27708
2University of Pittsburgh, School of Pharmacy, Magee Womens Research Institute, Pittsburgh, Pennsylvania 15213
3Duke University, Department of Biomedical Engineering, Center for Biomolecular and Tissue Engineering, 136 Hudson Hall, Box 90281, Durham, North Carolina 27708
Quantitative phase microscopy is applied to image temporal changes in the refractive index (RI) distributions of solutions created by microbicidal films undergoing hydration. We present a novel method of using an engineered polydimethylsiloxane structure as a static phase reference to facilitate calibration of the absolute RI across the entire field. We present a study of dynamic structural changes in microbicidal films during hydration and subsequent dissolution. With assumptions about the smoothness of the phase changes induced by these films, we calculate absolute changes in the percentage of film in regions across the field of view. ©2011 Society of Photo-Optical Instrumentation Engineers (SPIE)
History: Received 26 September 2011; revised 9 November 2011; accepted 14 November 2011; published 19 December 2011
Digital Object Identifier: http://dx.doi.org/10.1117/1.3665439

REFERENCES (15)

  1. A. Stone and P. F. Harrison, Microbicides: Ways Forward, Alliance for Microbicide Development, Silver Spring, MD (2010).
  2. S. Garg, D. Goldman, M. Krumme, L. C. Rohan, S. Smoot, and D. R. Friend, “Advances in development, scale-up and manufacturing of microbicide gels, films, and tablets,” Antiviral Rese. 88, S19–S29 (2010).
  3. N. T. Shaked, Y. Zhu, M. T. Rinehart, and A. Wax, “Two-step-only phase-shifting interferometry with optimized detector bandwidth for microscopy of live cells,” Opt. Express 17, 15585–15591 (2009).
  4. N. T. Shaked, M. T. Rinehart, and A. Wax, “Dual-interference-channel quantitative-phase microscopy of live cell dynamics,” Opt. Lett. 34, 767–769 (2009). [MEDLINE]
  5. B. Rappaz, F. Charrière, C. Depeursinge, P. J. Magistretti, and P. Marquet, “Simultaneous cell morphometry and refractive index measurement with dual-wavelength digital holographic microscopy and dye-enhanced dispersion of perfusion medium,” Opt. Lett. 33, 744–746 (2008). [MEDLINE]
  6. B. Rappaz, P. Marquet, E. Cuche, Y. Emery, C. Depeursinge, and P. J. Magistretti, “Measurement of the integral refractive index and dynamic cell morphometry of living cells with digital holographic microscopy,” Opt. Express 13, 9361–9373 (2005).
  7. B. Kemper, S. Kosmeier, P. Langehanenberg, G. von Bally, I. Bredebusch, W. Domschke, and J. Schnekenburger, “Integral refractive index determination of living suspension cells by multifocus digital holographic phase contrast microscopy,” J. Biomed. Opt. 12, 054009 (2007). [MEDLINE]
  8. N. Lue, G. Popescu, T. Ikeda, R. R. Dasari, K. Badizadegan, and M. S. Feld, “Live cell refractometry using microfluidic devices,” Opt. Lett. 31, 2759–2761 (2006). [MEDLINE]
  9. M. T. Rinehart, N. T. Shaked, N. J. Jenness, R. L. Clark, and A. Wax, “Simultaneous two-wavelength transmission quantitative phase microscopy with a color camera,” Opt. Lett. 35, 2612–2614 (2010).
  10. J. M. Huntley and H. Saldner, “Temporal phase-unwrapping algorithm for automated interferogram analysis,” Appl. Opt. 32, 3047–3052 (1993). [ISI]
  11. D. Ghiglia and M. Pritt, Two Dimensional Phase Unwrapping: Theory Algorithms & Software, Wiley, Hoboken, NJ (1998).
  12. P. Schiebener, J. Straub, J. M. H. Levelt Sengers, and J. S. Gallagher, “Refractive index of water and steam as function of wavelength, temperature and density,” J. Phys. Chem. Ref. Data 19, 677–717 (1990).
  13. J. Rheims, J. Köser, and T. Wriedt, “Refractive-index measurements in the near-IR using an Abbe refractometer,” Meas. Sci. Technol. 8, 601–605 (1997).
  14. L. F. Hoyt, “New table of the refractive index of pure glycerol at 20°C,” Ind. Eng. Chem. 26, 329–332 (1934).
  15. F. E. Robles, L. L. Satterwhite, and A. Wax, “Non-linear phase dispersion spectroscopy,” Opt. Lett. 36(23), 4665–4667 (2011).
ADVERTISEMENT