Biointerphases, Vol. 2, No. 4, pp. 119–125, December 2007
©2007 American Vacuum Society. All rights reserved.
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Direct observation of interaction between proteins and blood-compatible polymer surfaces

Tomohiro Hayashi*

Department of Electronic Chemistry, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8502, Japan and Local Spatio-Temporal Functions Laboratory, Frontier Research System, RIKEN (The Institute of Physical and Chemical Research), 2-1 Hirosawa, Wako, Saitama 351-0198, Japan

Masaru Tanaka†

2Nanotechnology Research Center, Research Institute for Electronic Science, Hokkaido University, N21W10, Sapporo, 001-0021, Japan

Sadaaki Yamamoto

Core Research Initiative “Sousei”, Hokkaido University, N21W10, Sapporo, 001-0021, Japan

Masatsugu Shimomura†

Nanotechnology Research Center, Research Institute for Electronic Science, Hokkaido University, N21W10, Sapporo, 001-0021, Japan

Masahiko Hara

Department of Electronic Chemistry, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8502, Japan and Local Spatio-Temporal Functions Laboratory, Frontier Research System, RIKEN (The Institute of Physical and Chemical Research), 2-1 Hirosawa, Wako, Saitama 351-0198, Japan

(Received: 26 February 2007; accepted: 12 September 2007; published online: 22 October 2007)

The adhesion force between blood-compatible polymer (poly(2-methoxyethyl acrylate: PMEA) and proteins (fibrinogen and bovine serum albumin (BSA)) were measured by atomic force microscopy. The PMEA surface showed almost no adhesion to native protein molecules, whereas non-blood-compatible poly(n-butyl acrylate): PBA strongly adhered to proteins. Interestingly, adhesion did appear between PMEA and proteins when the proteins were denatured. In all cases, these trends were not affected by the conditions of the solution. Combining the results with previous reports, the authors conclude that interfacial water molecules play a critical role in the protein resistance of PMEA. ©2007 American Vacuum Society

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