Biomicrofluidics http://scitation.aip.org/ Biomicrofluidics A new fabrication technique to form complex polymethylmethacrylate microchannel for bioseparation http://link.aip.org/link/?BMF/6/016503/1&agg=rss Talukder Z. Jubery, Mohammad R. Hossan, Danny R. Bottenus, Cornelius F. Ivory, Wenji Dong et al.<br/> Recent studies show that reduction in cross-sectional area can be used to improve the concentration factor in microscale bioseparations. Due to simplicity in fabrication process, a step reduction in cross-sectional area is generally implemented in microchip to increase the concentration factor. But ... [Biomicrofluidics 6, 016503 (2012)] published Fri Feb 10, 2012. Wafer-scale fabrication of high-aspect ratio nanochannels based on edge-lithography technique http://link.aip.org/link/?BMF/6/016502/1&agg=rss Quan Xie, Qing Zhou, Fei Xie, Jianming Sang, Wei Wang et al.<br/> This paper introduced a wafer-scale fabrication approach for the preparation of nanochannels with high-aspect ratio (the ratio of the channel depth to its width). Edge lithography was used to pattern nanogaps in an aluminum film, which was functioned as deep reactive ion etching mask thereafter to f ... [Biomicrofluidics 6, 016502 (2012)] published Thu Feb 9, 2012. High-throughput study of alpha-synuclein expression in yeast using microfluidics for control of local cellular microenvironment http://link.aip.org/link/?BMF/6/014109/1&agg=rss Patricia Rosa, Sandra Tenreiro, Virginia Chu, Tiago F. Outeiro, and Joao Pedro Conde<br/> Microfluidics is an emerging technology which allows the miniaturization, integration, and automation of fluid handling processes. Microfluidic systems offer low sample consumption, significantly reduced processing time, and the prospect of massive parallelization. A microfluidic platform was develo ... [Biomicrofluidics 6, 014109 (2012)] published Thu Feb 9, 2012. Michaelis-Menten kinetics in shear flow: Similarity solutions for multi-step reactions http://link.aip.org/link/?BMF/6/014108/1&agg=rss W. D. Ristenpart and H. A. Stone<br/> Models for chemical reaction kinetics typically assume well-mixed conditions, in which chemical compositions change in time but are uniform in space. In contrast, many biological and microfluidic systems of interest involve non-uniform flows where gradients in flow velocity dynamically alter the eff ... [Biomicrofluidics 6, 014108 (2012)] published Tue Jan 31, 2012. A tapered channel microfluidic device for comprehensive cell adhesion analysis, using measurements of detachment kinetics and shear stress-dependent motion http://link.aip.org/link/?BMF/6/014107/1&agg=rss Peter Rupprecht, Laurent Gole, Jean-Paul Rieu, Cyrille Vezy, Rosaria Ferrigno et al.<br/> We have developed a method for studying cellular adhesion by using a custom-designed microfluidic device with parallel non-connected tapered channels. The design enables investigation of cellular responses to a large range of shear stress (ratio of 25) with a single input flow-rate. For each shear s ... [Biomicrofluidics 6, 014107 (2012)] published Tue Jan 31, 2012. Fluid flow due to collective non-reciprocal motion of symmetrically-beating artificial cilia http://link.aip.org/link/?BMF/6/014106/1&agg=rss S. N. Khaderi, J. M. J. den Toonder, and P. R. Onck<br/> Using a magneto-mechanical solid-fluid numerical model for permanently magnetic artificial cilia, we show that the metachronal motion of symmetrically beating cilia establishes a net pressure gradient in the direction of the metachronal wave, which creates a unidirectional flow. The flow generated i ... [Biomicrofluidics 6, 014106 (2012)] published Fri Jan 20, 2012. A nanoporous optofluidic microsystem for highly sensitive and repeatable surface enhanced Raman spectroscopy detection http://link.aip.org/link/?BMF/6/014105/1&agg=rss Soroush H. Yazdi and Ian M. White<br/> We report the demonstration of an optofluidic surface enhanced Raman spectroscopy (SERS) device that leverages a nanoporous microfluidic matrix to improve the SERS detection performance by more than two orders of magnitude as compared to a typical open microfluidic channel. Although it is a growing ... [Biomicrofluidics 6, 014105 (2012)] published Fri Jan 13, 2012. Microfluidic carbon-blackened polydimethylsiloxane device with reduced ultra violet background fluorescence for simultaneous two-color ultra violet/visible-laser induced fluorescence detection in single cell analysis http://link.aip.org/link/?BMF/6/014104/1&agg=rss Lukas Galla, Dominik Greif, Jan Regtmeier, and Dario Anselmetti<br/> In single cell analysis (SCA), individual cell-specific properties and inhomogeneous cellular responses are being investigated that is not subjected to ensemble-averaging or heterogeneous cell population effects. For proteomic single cell analysis, ultra-sensitive and reproducible separation and det ... [Biomicrofluidics 6, 014104 (2012)] published Thu Jan 12, 2012. Resistive pulse sensing of magnetic beads and supraparticle structures using tunable pores http://link.aip.org/link/?BMF/6/014103/1&agg=rss Geoff R. Willmott, Mark Platt, and Gil U. Lee<br/> Tunable pores (TPs) have been used for resistive pulse sensing of 1 [mu]m superparamagnetic beads, both dispersed and within a magnetic field. Upon application of this field, magnetic supraparticle structures (SPSs) were observed. Onset of aggregation was most effectively indicated by an increase in ... [Biomicrofluidics 6, 014103 (2012)] published Thu Jan 12, 2012. Electrotaxis of lung cancer cells in ordered three-dimensional scaffolds http://link.aip.org/link/?BMF/6/014102/1&agg=rss Yung-Shin Sun, Shih-Wei Peng, Keng-Hui Lin, and Ji-Yen Cheng<br/> In this paper, we report a new method to incorporate 3D scaffold with electrotaxis measurement in the microfluidic device. The electrotactic response of lung cancer cells in the 3D foam scaffolds which resemble the in vivo pulmonary alveoli may give more insight on cellular behaviors in vivo. The 3D ... [Biomicrofluidics 6, 014102 (2012)] published Wed Jan 4, 2012. Oxygen and nitrogen plasma hydrophilization and hydrophobic recovery of polymers http://link.aip.org/link/?BMF/6/016501/1&agg=rss Ville Jokinen, Pia Suvanto, and Sami Franssila<br/> Plasma hydrophilization and subsequent hydrophobic recovery are studied for ten different polymers of microfabrication interest: polydimethylsiloxane (PDMS), polymethylmethacrylate, polycarbonate, polyethylene, polypropylene, polystyrene, epoxy polymer SU-8, hybrid polymer ORMOCOMP, polycaprolactone ... [Biomicrofluidics 6, 016501 (2012)] published Tue Jan 3, 2012. Resolution limit for DNA barcodes in the Odijk regime http://link.aip.org/link/?BMF/6/014101/1&agg=rss Yanwei Wang, Wes F. Reinhart, Douglas R. Tree, and Kevin D. Dorfman<br/> We develop an approximation for the probability of optically resolving two fluorescent labels on the backbone of a DNA molecule confined in a nanochannel in the Odijk regime as a function of the fluorescence wavelength, channel size, and the properties of the DNA (persistence length and effective wi ... [Biomicrofluidics 6, 014101 (2012)] published Tue Jan 3, 2012. Asymmetry of red blood cell motions in a microchannel with a diverging and converging bifurcation http://link.aip.org/link/?BMF/5/044120/1&agg=rss Vladimir Leble, Rui Lima, Ricardo Dias, Carla Fernandes, Takuji Ishikawa et al.<br/> In microcirculation, red blood cells (RBCs) flowing through bifurcations may deform considerably due to combination of different phenomena that happen at the micro-scale level, such as: attraction effect, high shear, and extensional stress, all of which may influence the rheological properties and f ... [Biomicrofluidics 5, 044120 (2011)] published Fri Dec 23, 2011. Effects of chain stiffness and salt concentration on responses of polyelectrolyte brushes under external electric field http://link.aip.org/link/?BMF/5/044119/1&agg=rss Qianqian Cao, Chuncheng Zuo, Lujuan Li, and Guang Yan<br/> We report a molecular dynamics study on non-equilibrium dynamics of polyelectrolyte brushes under external electric fields. In this work, the effects of chain stiffness and salt concentration on static and dynamic responses of the brushes are addressed in detail. Our simulations indicate that varyin ... [Biomicrofluidics 5, 044119 (2011)] published Wed Dec 21, 2011. Growth propagation of yeast in linear arrays of microfluidic chambers over many generations http://link.aip.org/link/?BMF/5/044118/1&agg=rss Li Wang, Jiaji Liu, Xin Li, Jian Shi, Jie Hu et al.<br/> The growth of microorganisms is often confined in restricting geometries. In this work, we designed a device to study the growth propagation of budding yeast along linear arrays of microfluidic chambers. Vacuum assisted cell loading was used to seed cells of limited numbers in the up-most chambers o ... [Biomicrofluidics 5, 044118 (2011)] published Fri Dec 16, 2011. Microwell perfusion array for high-throughput, long-term imaging of clonal growth http://link.aip.org/link/?BMF/5/044117/1&agg=rss Huaying Chen, Jingjing Li, Han Zhang, Musen Li, Gary Rosengarten et al.<br/> Continuous cell tracking by time-lapse microscopy has led to detailed study of cell differentiation pathways using single cell fate maps. There are a multitude of cell fate outcomes, so hundreds of clonal division histories are required to measure these stochastic branching processes. This study exa ... [Biomicrofluidics 5, 044117 (2011)] published Thu Dec 15, 2011. Effect of pulse direct current signals on electrotactic movement of nematodes Caenorhabditis elegans and Caenorhabditis briggsae http://link.aip.org/link/?BMF/5/044116/1&agg=rss Pouya Rezai, Sangeena Salam, Ponnambalam Ravi Selvaganapathy, and Bhagwati P. Gupta<br/> The nematodes (worms) Caenorhabditis elegans and Caenorhabditis briggsae are well-known model organisms to study the basis of animal development and behaviour. Their sinusoidal pattern of movement is highly stereotypic and serves as a tool to monitor defects in neurons and muscles that control movem ... [Biomicrofluidics 5, 044116 (2011)] published Thu Dec 15, 2011. A parallel microfluidic channel fixture fabricated using laser ablated plastic laminates for electrochemical and chemiluminescent biodetection of DNA http://link.aip.org/link/?BMF/5/044115/1&agg=rss Thayne L. Edwards, Jason C. Harper, Ronen Polsky, DeAnna M. Lopez, David R. Wheeler et al.<br/> Herein is described the fabrication and use of a plastic multilayer 3-channel microfluidic fixture. Multilayer devices were produced by laser machining of plastic polymethylmethacrylate and polyethyleneterapthalate laminates by ablation. The fixture consisted of an array of nine individually address ... [Biomicrofluidics 5, 044115 (2011)] published Thu Dec 15, 2011. Three-dimensional fit-to-flow microfluidic assembly http://link.aip.org/link/?BMF/5/046505/1&agg=rss Arnold Chen and Tingrui Pan<br/> Three-dimensional microfluidics holds great promise for large-scale integration of versatile, digitalized, and multitasking fluidic manipulations for biological and clinical applications. Successful translation of microfluidic toolsets to these purposes faces persistent technical challenges, such as ... [Biomicrofluidics 5, 046505 (2011)] published Wed Dec 14, 2011. The effects of laser welding on heterogeneous immunoassay performance in a microfluidic cartridge http://link.aip.org/link/?BMF/5/046504/1&agg=rss Anne Mantymaa, Jussi Halme, Lasse Valimaa, and Pasi Kallio<br/> Sealing of a microfluidic cartridge is a challenge, because the cartridge commonly contains heat-sensitive biomolecules that must also be protected from contamination. In addition, the objective is usually to obtain a sealing method suitable for mass production. Laser welding is a rapid technique th ... [Biomicrofluidics 5, 046504 (2011)] published Mon Dec 12, 2011. Characterization of low viscosity polymer solutions for microchip electrophoresis of non-denatured proteins on plastic chips http://link.aip.org/link/?BMF/5/044114/1&agg=rss Takao Yasui, Mohamad Reza Mohamadi, Noritada Kaji, Yukihiro Okamoto, Manabu Tokeshi et al.<br/> In this paper, we study characteristics of polymers (methylcellulose, hypromellose ((hydroxypropyl)methyl cellulose), poly(vinylpyrrolidone), and poly(vinyl alcohol)) with different chemical structures for microchip electrophoresis of non-denatured protein samples in a plastic microchip made of poly ... [Biomicrofluidics 5, 044114 (2011)] published Mon Dec 12, 2011.