Biomicrofluidics http://scitation.aip.org/ Biomicrofluidics Direct detection of cancer biomarkers in blood using a place n play modular polydimethylsiloxane pump http://link.aip.org/link/?BMF/7/034105/1&agg=rss Honglian Zhang, Gang Li, Lingying Liao, HongJu Mao, Qinghui Jin et al.<br/> Cancer biomarkers have significant potential as reliable tools for the early detection of the disease and for monitoring its recurrence. However, most current methods for biomarker detection have technical difficulties (such as sample preparation and specific detector requirements) which limit their ... [Biomicrofluidics 7, 034105 (2013)] published Thu May 23, 2013. Continuous flowing micro-reactor for aqueous reaction at temperature higher than 100 degrees C http://link.aip.org/link/?BMF/7/034104/1&agg=rss Fei Xie, Baojun Wang, Wei Wang, Tian Dong, Jianhua Tong et al.<br/> Some aqueous reactions in biological or chemical fields are accomplished at a high temperature. When the reaction temperature is higher than 100 degrees C, an autoclave reactor is usually required to elevate the boiling point of the water by creating a high-pressure environment in a closed system. ... [Biomicrofluidics 7, 034104 (2013)] published Tue May 21, 2013. Simulation of single DNA molecule stretching and immobilization in a de-wetting two-phase flow over micropillar-patterned surface http://link.aip.org/link/?BMF/7/034103/1&agg=rss Wei-Ching Liao, Xin Hu, Weixiong Wang, and L. James Lee<br/> We investigate single DNA stretching dynamics in a de-wetting flow over micropillars using Brownian dynamics simulation. The Brownian dynamics simulation is coupled with transient flow field computation through a numerical particle tracking algorithm. The droplet formation on the top of the micropil ... [Biomicrofluidics 7, 034103 (2013)] published Tue May 21, 2013. Viscoelasticity of blood and viscoelastic blood analogues for use in polydymethylsiloxane in vitro models of the circulatory system http://link.aip.org/link/?BMF/7/034102/1&agg=rss Laura Campo-Deano, Roel P. A. Dullens, Dirk G. A. L. Aarts, Fernando T. Pinho, and Monica S. N. Oliveira<br/> The non-Newtonian properties of blood are of great importance since they are closely related with incident cardiovascular diseases. A good understanding of the hemodynamics through the main vessels of the human circulatory system is thus fundamental in the detection and especially in the treatment o ... [Biomicrofluidics 7, 034102 (2013)] published Fri May 17, 2013. Ionic current devicesRecent progress in the merging of electronic, microfluidic, and biomimetic structures http://link.aip.org/link/?BMF/7/031501/1&agg=rss Hyung-Jun Koo and Orlin D. Velev<br/> We review the recent progress in the emerging area of devices and circuits operating on the basis of ionic currents. These devices operate at the intersection of electrochemistry, electronics, and microfluidics, and their potential applications are inspired by essential biological processes such as ... [Biomicrofluidics 7, 031501 (2013)] published Thu May 9, 2013. A hybrid microfluidic platform for cell-based assays via diffusive and convective trans-membrane perfusion http://link.aip.org/link/?BMF/7/034101/1&agg=rss Elizaveta Vereshchagina, Declan Mc Glade, Macdara Glynn, and Jens Ducree<br/> We present a novel 3D hybrid assembly of a polymer microfluidic chip with polycarbonate track-etched membrane (PCTEM) enabling membrane-supported cell culture. Two chip designs have been developed to establish either diffusive or convective reagent delivery using the integrated PCTEM. While it is we ... [Biomicrofluidics 7, 034101 (2013)] published Wed May 8, 2013. Single cell rheometry with a microfluidic constriction: Quantitative control of friction and fluid leaks between cell and channel walls http://link.aip.org/link/?BMF/7/024111/1&agg=rss Pascal Preira, Marie-Pierre Valignat, Jose Bico, and Olivier Theodoly<br/> We report how cell rheology measurements can be performed by monitoring the deformation of a cell in a microfluidic constriction, provided that friction and fluid leaks effects between the cell and the walls of the microchannels are correctly taken into account. Indeed, the mismatch between the roun ... [Biomicrofluidics 7, 024111 (2013)] published Tue Apr 23, 2013. Protein sensing by nanofluidic crystal and its signal enhancement http://link.aip.org/link/?BMF/7/024112/1&agg=rss Jianming Sang, Hongtan Du, Wei Wang, Ming Chu, Yuedan Wang et al.<br/> Nanofluidics has a unique property that ionic conductance across a nanometer-sized confined space is strongly affected by the space surface charge density, which can be utilized to construct electrical read-out biosensor. Based on this principle, this work demonstrated a novel protein sensor along w ... [Biomicrofluidics 7, 024112 (2013)] published Tue Apr 23, 2013. A fluorescence based method for the quantification of surface functional groups in closed micro- and nanofluidic channels http://link.aip.org/link/?BMF/7/026503/1&agg=rss Yu Wang, Rachel D. Lowe, Yara X. Mejia, Holger Feindt, Siegfried Steltenkamp et al.<br/> Surface analysis is critical for the validation of microfluidic surface modifications for biology, chemistry, and physics applications. However, until now quantitative analytical methods have mostly been focused on open surfaces. Here, we present a new fluorescence imaging method to directly measure ... [Biomicrofluidics 7, 026503 (2013)] published Mon Apr 22, 2013. A capillary dielectrophoretic chip for real-time blood cell separation from a drop of whole blood http://link.aip.org/link/?BMF/7/024110/1&agg=rss Shu-Hsien Liao, Ching-Yu Chang, and Hsien-Chang Chang<br/> This study proposes a capillary dielectrophoretic chip to separate blood cells from a drop of whole blood (approximately 1 [mu]l) sample using negative dielectrophoretic force. The separating efficiency was evaluated by analyzing the image before and after dielectrophoretic force manipulation. Blood ... [Biomicrofluidics 7, 024110 (2013)] published Thu Apr 18, 2013. Alternating current-dielectrophoresis driven on-chip collection and chaining of green microalgae in freshwaters http://link.aip.org/link/?BMF/7/024109/1&agg=rss Coralie Suscillon, Orlin D. Velev, and Vera I. Slaveykova<br/> The capability of the AC dielectrophoresis (DEP) for on-chip capture and chaining of microalgae suspended in freshwaters was evaluated. The effects of freshwater composition as well as the electric field voltage, frequency, and duration, on the dielectrophoretic response of microalga Chlamydomonas r ... [Biomicrofluidics 7, 024109 (2013)] published Tue Apr 16, 2013. Block-and-break generation of microdroplets with fixed volume http://link.aip.org/link/?BMF/7/024108/1&agg=rss Volkert van Steijn, Piotr M. Korczyk, Ladislav Derzsi, Adam R. Abate, David A. Weitz et al.<br/> We introduce a novel type of droplet generator that produces droplets of a volume set by the geometry of the droplet generator and not by the flow rates of the liquids. The generator consists of a classic T-junction with a bypass channel. This bypass directs the continuous fluid around the forming d ... [Biomicrofluidics 7, 024108 (2013)] published Wed Apr 10, 2013. Review of microfluidic microbioreactor technology for high-throughput submerged microbiological cultivation http://link.aip.org/link/?BMF/7/021502/1&agg=rss Hanaa M. Hegab, Ahmed ElMekawy, and Tim Stakenborg<br/> Microbial fermentation process development is pursuing a high production yield. This requires a high throughput screening and optimization of the microbial strains, which is nowadays commonly achieved by applying slow and labor-intensive submerged cultivation in shake flasks or microtiter plates. Th ... [Biomicrofluidics 7, 021502 (2013)] published Fri Apr 5, 2013. Hydrodynamic mechanisms of cell and particle trapping in microfluidics http://link.aip.org/link/?BMF/7/021501/1&agg=rss A. Karimi, S. Yazdi, and A. M. Ardekani<br/> Focusing and sorting cells and particles utilizing microfluidic phenomena have been flourishing areas of development in recent years. These processes are largely beneficial in biomedical applications and fundamental studies of cell biology as they provide cost-effective and point-of-care miniaturize ... [Biomicrofluidics 7, 021501 (2013)] published Fri Apr 5, 2013. Efficient sample preparation in immuno-matrix-assisted laser desorption/ionization mass spectrometry using acoustic trapping http://link.aip.org/link/?BMF/7/024107/1&agg=rss Bjorn Hammarstrom, Hong Yan, Johan Nilsson, and Simon Ekstrom<br/> Acoustic trapping of minute bead amounts against fluid flow allows for easy automation of multiple assay steps, using a convenient aspirate/dispense format. Here, a method based on acoustic trapping that allows sample preparation for immuno-matrix-assisted laser desorption/ionization mass spectromet ... [Biomicrofluidics 7, 024107 (2013)] published Thu Mar 28, 2013. Development of vertical SU-8 microtubes integrated with dissolvable tips for transdermal drug delivery http://link.aip.org/link/?BMF/7/026502/1&agg=rss Zhuolin Xiang, Hao Wang, Aakanksha Pant, Giorgia Pastorin, and Chengkuo Lee<br/> Polymer-based microneedles have drawn much attention in the transdermal drug delivery resulting from their flexibility and biocompatibility. Traditional fabrication approach deploys various kinds of molds to create sharp tips at the end of needles for the penetration purpose. This approach is usuall ... [Biomicrofluidics 7, 026502 (2013)] published Tue Mar 26, 2013. High-throughput particle manipulation by hydrodynamic, electrokinetic, and dielectrophoretic effects in an integrated microfluidic chip http://link.aip.org/link/?BMF/7/024106/1&agg=rss Shunbo Li, Ming Li, Kristelle Bougot-Robin, Wenbin Cao, Irene Yeung Yeung Chau et al.<br/> Integrating different steps on a chip for cell manipulations and sample preparation is of foremost importance to fully take advantage of microfluidic possibilities, and therefore make tests faster, cheaper and more accurate. We demonstrated particle manipulation in an integrated microfluidic device ... [Biomicrofluidics 7, 024106 (2013)] published Wed Mar 20, 2013. Reconfigurable microfluidics combined with antibody microarrays for enhanced detection of T-cell secreted cytokines http://link.aip.org/link/?BMF/7/024105/1&agg=rss Arnold Chen, Tam Vu, Gulnaz Stybayeva, Tingrui Pan, and Alexander Revzin<br/> Cytokines are small proteins secreted by leukocytes in blood in response to infections, thus offering valuable diagnostic information. Given that the same cytokines may be produced by different leukocyte subsets in blood, it is beneficial to connect production of cytokines to specific cell types. In ... [Biomicrofluidics 7, 024105 (2013)] published Thu Mar 14, 2013. Review article: Fabrication of nanofluidic devices http://link.aip.org/link/?BMF/7/026501/1&agg=rss Chuanhua Duan, Wei Wang, and Quan Xie<br/> Thanks to its unique features at the nanoscale, nanofluidics, the study and application of fluid flow in nanochannels/nanopores with at least one characteristic size smaller than 100 nm, has enabled the occurrence of many interesting transport phenomena and has shown great potential in both bio- and ... [Biomicrofluidics 7, 026501 (2013)] published Wed Mar 13, 2013. A numerical study on distributions during cryoprotectant loading caused by laminar flow in a microchannel http://link.aip.org/link/?BMF/7/024104/1&agg=rss T. Scherr, S. Pursley, W. T. Monroe, and K. Nandakumar<br/> In this work, we conduct a computational study on the loading of cryoprotective agents into cells in preparation for cryopreservation. The advantages of microfluidics in cryopreserving cells include control of fluid flow parameters for reliable cryoprotectant loading and reproducible streamlined pro ... [Biomicrofluidics 7, 024104 (2013)] published Mon Mar 11, 2013. A negative-pressure-driven microfluidic chip for the rapid detection of a bladder cancer biomarker in urine using bead-based enzyme-linked immunosorbent assay http://link.aip.org/link/?BMF/7/024103/1&agg=rss Yen-Heng Lin, Ying-Ju Chen, Chao-Sung Lai, Yi-Ting Chen, Chien-Lun Chen et al.<br/> This paper describes an integrated microfluidic chip that is capable of rapidly and quantitatively measuring the concentration of a bladder cancer biomarker, apolipoprotein A1, in urine samples. All of the microfluidic components, including the fluid transport system, the micro-valve, and the micro- ... [Biomicrofluidics 7, 024103 (2013)] published Thu Mar 7, 2013.